ISDA: 30LF

Deposition Date 2026-05-01

Release Date 2026-10-28

Last Version Date 2026-10-28

Entry Detail

PDB ID:

30LF

Keywords:

ISOMERASE

Title:

2.3 A structure of the NBTI B1 with S. aureus DNA gyrase and double-nicked DNA

Biological Source:

Source Organism(s):

Staphylococcus aureus subsp. aureus N315 (Taxon ID: 158879)
synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

2.30 Å

R-Value Free:

0.25

R-Value Work:

0.21

Space Group:

P 61

Download Validation Report

Macromolecular Entities

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA gyrase subunit B,DNA gyra
Gene (Uniprot):gyrB, gyrA
Chain IDs:A, B
Chain Length:692
Number of Molecules:2
Biological Source:Staphylococcus aureus subsp. aureus N315

UniProt ID:P66937,Q99XG5 EC:5.6.2.2

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(*AP*GP*CP*CP*GP*TP*
Chain IDs:C (auth: E), D (auth: G)
Chain Length:8
Number of Molecules:2
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(P*GP*TP*AP*CP*CP*TP
Chain IDs:E (auth: F), F (auth: H)
Chain Length:12
Number of Molecules:2
Biological Source:synthetic construct

Ligand Molecules