ISDA: 28JJ

Deposition Date 2026-02-03

Release Date 2026-07-22

Last Version Date 2026-07-22

Entry Detail

PDB ID:

28JJ

Keywords:

OXIDOREDUCTASE

Title:

Crystal structure of rat peroxisomal multifunctional enzyme type-1 complexed with 2E,4E-decadienoyl-CoA, 3R-hydroxy-4E-decenoyl-CoA and NAD

Biological Source:

Source Organism(s):

Rattus norvegicus (Taxon ID: 10116)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

2.30 Å

R-Value Free:

0.24

R-Value Work:

0.22

Space Group:

P 21 21 21

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Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Peroxisomal bifunctional enzy
Gene (Uniprot):Ehhadh
Chain IDs:A, B
Chain Length:742
Number of Molecules:2
Biological Source:Rattus norvegicus

UniProt ID:P07896 Pfam ID:PF00378, PF02737, PF00725 EC:4.2.1.17

Ligand Molecules

A1JZ1

A1JZN

GOL

NAD

SO4

Primary Citation

Structural enzymological studies of multifunctional enzyme, type-1 (MFE1) with the 2E-decenoyl-CoA and 2E,4E-decadienoyl-CoA substrates: The regeneration of the dehydrogenase catalytic site is the rate limiting step of its combined reactions.

Sridhar, S, Schmitz, W, Widersten, M, Wierenga, R.K, Kiema, T.R.Show

J.Struct.Biol. ? 108346 108346 (2026)

PMID: 42435994 DOI: 10.1016/j.jsb.2026.108346

Primary Citation of related structures

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