ELECTRON MICROSCOPY

Sample

Wild-type human CFTR solubilized in digitonin and cholesterol-hemisuccinate in the presence of 2 mM MgATP

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 81674
Reported Resolution (Å) 3.14
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details Real space refinement in PHENIX v 2.1_6048
Data Acquisition
Detector Type GATAN K3 BIOQUANTUM (6k x 4k)
Electron Dose (electrons/Å2) 58.0
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 200
Maximum Defocus (nm) 2900
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 105000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4.3.1
IMAGE ACQUISITION Leginon 3.6
CTF CORRECTION cryoSPARC 4.3.1
MODEL REFINEMENT PHENIX 2.1_6048
INITIAL EULER ASSIGNMENT cryoSPARC 4.3.1
FINAL EULER ASSIGNMENT cryoSPARC 4.3.1
CLASSIFICATION cryoSPARC 4.7.0
RECONSTRUCTION cryoSPARC 4.7.0
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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