ISDA: 9SVH

ELECTRON MICROSCOPY

Sample

Complex of CRL4 E3 adapter-receptor pair DDB1 and CRBN bound to 1-[1-(4-piperidyl)indol-4-yl]hexahydropyrimidine-2,4-dione

Specimen Preperation

Sample Aggregation State	PARTICLE
Vitrification Instrument	?
Cryogen Name	ETHANE
Sample Vitrification Details	?

3D Reconstruction

Reconstruction Method	SINGLE PARTICLE
Number of Particles	56510
Reported Resolution (Å)	3.66
Resolution Method	FSC 0.143 CUT-OFF
Other Details	cryoSPARC non-uniform refinement was used for the final reconstruction of the consensus map, and cryoSPARC local refinement was used for the locally refined map of the LON and thalidomide binding domains
Refinement Type
Symmetry Type	POINT

Map-Model Fitting and Refinement

ID	1
Refinement Space	REAL
Refinement Protocol	FLEXIBLE FIT
Refinement Target	?
Overall B Value	?
Fitting Procedure	?
Details	Initial fitting was done in ChimeraX, followed by flexible fitting in Coot. The model was refined with iterative rounds of building in Coot and refinement in PHENIX real space refine

Data Acquisition

Detector Type	TFS FALCON 4i (4k x 4k)
Electron Dose (electrons/Å²)	21.94

Imaging Experiment

Date of Experiment	?
Temprature (Kelvin)
Microscope Model	TFS GLACIOS
Minimum Defocus (nm)	1700
Maximum Defocus (nm)	3200
Minimum Tilt Angle (degrees)	?
Maximum Tilt Angle (degrees)	?
Nominal CS	?
Imaging Mode	BRIGHT FIELD
Specimen Holder Model	FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification	190000
Calibrated Magnification	?
Source	FIELD EMISSION GUN
Acceleration Voltage (kV)	200
Imaging Details	?

Imaging Experiment

Image Processing

CTF Correction Type	CTF Correction Details	Number of Particles Selected	Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION	Patch CTF