ELECTRON MICROSCOPY

Sample

43S pre-initiation complex consisting of the 40S small ribosome subunit, eIF1, eIF1A, eIF2, eIF3 and initiator tRNA bound to circMbl-FLAG

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK II
Cryogen Name ETHANE
Sample Vitrification Details Wait time: 30 s Blot force: -3 Blot time: 4-5 s
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 21721
Reported Resolution (Å) 3.1
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol FLEXIBLE FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 BIOQUANTUM (6k x 4k)
Electron Dose (electrons/Å2) 40.0
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 1200
Maximum Defocus (nm) 2800
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification 105000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION RELION 5.0
MODEL REFINEMENT PHENIX 1.21.2_5419
IMAGE ACQUISITION EPU ?
CTF CORRECTION CTFFIND 4.1
INITIAL EULER ASSIGNMENT RELION 5.0
FINAL EULER ASSIGNMENT RELION 5.0
CLASSIFICATION RELION 5.0
RECONSTRUCTION RELION 5.0
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
NONE ?
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