ISDA: 9O47

X-RAY DIFFRACTION

Crystallization Details

Method	pH	Temprature	Details
X-RAY DIFFRACTION	6.0	293.15	Drops contained 400 nanoliters of 1 mM RNA and 400 nanoliters of buffer containing 10% 2-methyl-2,4- pentanediol (MPD), 12 mM spermine 4 HCl, 80 mM potassium chloride, and 40 mM sodium cacodylate, pH 6.0. Drops were equilibrated against reservoirs containing 70 microliters of 40% MPD

Unit Cell:

a: 24.021 Å b: 29.185 Å c: 29.782 Å α: 110.874° β: 100.287° γ: 111.585°

Symmetry:

Space Group: P 1

Crystal Properties:

Matthew's Coefficient: 2.19 Solvent Content: 43.85

Refinement Statistics

Diffraction ID	Structure Solution Method	Cross Validation Method	Resolution Limit (High)	Resolution Limit (Low)	Number of Reflections (Observed)	Number of Reflections (R-free)	Percent Reflections (Observed)	R-Work	R-Free	Mean Isotropic
X-RAY DIFFRACTION	SAD	FREE R-VALUE	1.60	16.24	16979	806	98.25	0.1972	0.2233	23.79

Data Collection

Overall

Resolution Limit (High)	Resolution Limit (Low)	Percent Possible (Observed)	R Merge I (Observed)	R Sym I (Observed)	Net I Over Average Sigma (I)	Redundancy	Number Reflections (All)	Number Reflections (Observed)	Observed Criterion Sigma (F)	Observed Criterion Sigma (I)	B (Isotropic) From Wilson Plot
1.6	16.24	98.25	?	?	3.4	2.9	?	8629	?	?	16.60

Highest Resolution Shell

#	Resolution Limits (Low)	Resolution Limits (High)	Percent Possible (All)	Percent Possible (Observed)	R-Sym I (Observed)	Mean I Over Sigma (Observed)	Redundancy	Number Unique Reflections (All)
1	1.6	1.7	?	?	?	?	?	?

Diffraction

Diffraction experiment

Crystal ID	Scattering Type	Data Collection Temprature	Detector	Detector Type	Details	Collection Date	Monochromator	Protocol
1		100 K			?

Radiation Source

Source	Type	Wavelength List	Synchrotron Site	Beamline
LIQUID ANODE	Excillum MetalJet D2+ 70 kV	1.342	?	?

Software