ISDA: 9ZHM

Deposition Date 2025-12-03

Release Date 2026-07-22

Last Version Date 2026-07-22

Entry Detail

PDB ID:

9ZHM

Keywords:

OXIDOREDUCTASE

Title:

M20E Mutant of E. coli Dihydrofolate Reductase Complexed with Nicotinamide Adenine Dinucleotide Phosphate (oxidized form)

Biological Source:

Source Organism(s):

Escherichia coli (Taxon ID: 562)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.07 Å

R-Value Free:

0.15

R-Value Work:

0.13

R-Value Observed:

0.13

Space Group:

P 21 21 21

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Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Dihydrofolate reductase
Gene (Uniprot):folA
Mutagens:M20E
Chain IDs:A
Chain Length:159
Number of Molecules:1
Biological Source:Escherichia coli

UniProt ID:P0ABQ4 Pfam ID:PF00186 EC:1.5.1.3

Ligand Molecules

FOL

NAP

Primary Citation

Role of Electrostatics in Hydride Transfer by Dihydrofolate Reductase

Fried, S.D.E, Mukherjee, S, Mathews, I.I, Boxer, S.G.Show

To Be Published ? ? ? (?)

Primary Citation of related structures

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