ISDA: 9ZE3

Deposition Date 2025-11-27

Release Date 2026-04-15

Last Version Date 2026-04-15

Entry Detail

PDB ID:

9ZE3

Keywords:

SPLICING

Title:

Cryo-EM structure of the endogenous U2/branchpoint spliceosomal complex (Distal DHX15 state)

Biological Source:

Source Organism(s):

Homo sapiens (Taxon ID: 9606)

Expression System(s):

Homo sapiens

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.93 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Polymer Type:polyribonucleotide
Molecule:U2 snRNA
Chain IDs:D (auth: 2)
Chain Length:37
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:RNA-binding protein 5
Chain IDs:A
Chain Length:824
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Splicing factor 3A subunit 1
Chain IDs:M (auth: A1)
Chain Length:793
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Splicing factor 3A subunit 2
Chain IDs:N (auth: A2)
Chain Length:464
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Splicing factor 3A subunit 3
Chain IDs:O (auth: A3)
Chain Length:501
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:ATP-dependent RNA helicase DH
Chain IDs:B
Chain Length:795
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Splicing factor 3B subunit 1
Chain IDs:G (auth: B1)
Chain Length:1304
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Splicing factor 3B subunit 2
Chain IDs:H (auth: B2)
Chain Length:895
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Splicing factor 3B subunit 3
Chain IDs:I (auth: B3)
Chain Length:1217
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Splicing factor 3B subunit 4
Chain IDs:J (auth: B4)
Chain Length:424
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Splicing factor 3B subunit 5
Chain IDs:K (auth: B5)
Chain Length:86
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:Splicing factor 3B subunit 6
Chain IDs:L (auth: B6)
Chain Length:125
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:U2 snRNP-associated SURP moti
Chain IDs:C
Chain Length:1029
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polypeptide(L)
Molecule:PHD finger-like domain-contai
Chain IDs:E (auth: H)
Chain Length:110
Number of Molecules:1
Biological Source:Homo sapiens

Polymer Type:polyribonucleotide
Molecule:Pre-mRNA
Chain IDs:F (auth: R)
Chain Length:44
Number of Molecules:1
Biological Source:Homo sapiens

Ligand Molecules

Primary Citation

The tumour suppressor RBM5 activates the helicase DHX15 to regulate splicing.

Liu, S, Su, T, Huang, J, Lin, C.H, Black, D.L, Damianov, A, Zhou, Z.H.Show

Biorxiv ? ? ? (2026)

PMID: 41929118 DOI: 10.64898/2026.03.26.714623

Primary Citation of related structures

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