ISDA: 9YZU

Deposition Date 2025-10-30

Release Date 2026-02-18

Last Version Date 2026-02-18

Entry Detail

PDB ID:

9YZU

Keywords:

DNA BINDING PROTEIN/DNA

Title:

Isoreticular co-crystal 1 with asymmetrical expanded duplex (31mer) containing insert sequence ATGAGTCATA and loaded with mutated Bzip region of GCN4 transcription factor

Biological Source:

Source Organism(s):

Escherichia coli (Taxon ID: 562)
Saccharomyces cerevisiae (Taxon ID: 4932)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

3.05 Å

R-Value Free:

0.24

R-Value Work:

0.22

R-Value Observed:

0.22

Space Group:

C 1 2 1

Download Validation Report

Macromolecular Entities

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (31-MER)
Chain IDs:A
Chain Length:31
Number of Molecules:1
Biological Source:Escherichia coli

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (31-MER)
Chain IDs:B
Chain Length:31
Number of Molecules:1
Biological Source:Escherichia coli

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Replication initiation protei
Gene (Uniprot):repE
Chain IDs:C
Chain Length:263
Number of Molecules:1
Biological Source:Escherichia coli

UniProt ID:P03856

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:General control transcription
Gene (Uniprot):GCN4
Chain IDs:D (auth: F), E (auth: D), F (auth: E)
Chain Length:29
Number of Molecules:3
Biological Source:Saccharomyces cerevisiae

UniProt ID:P03069 Pfam ID:PF07716

Ligand Molecules

Primary Citation

Modular Scaffold Crystals for Programmable Installation and Structural Observation of DNA-Binding Proteins

Shields, E.T, Slaughter, C.K, Mekkaoui, F, Magna, E.N, Shepherd, C, Lukeman, P.S, Spratt, D.E, Snow, C.D.Show

To Be Published ? ? ? (?)

Primary Citation of related structures

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