ISDA: 9YD1

Deposition Date 2025-09-20

Release Date 2026-06-24

Last Version Date 2026-07-15

Entry Detail

PDB ID:

9YD1

Keywords:

REPLICATION

Title:

Crystal structure of BF DNA polymerase F710Y mutant bound to tetrahydrofuran and dATP

Biological Source:

Source Organism(s):

Geobacillus stearothermophilus (Taxon ID: 1422)
synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli 'bl21-gold(de3)plyss ag'

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.80 Å

R-Value Free:

0.24

R-Value Work:

0.20

R-Value Observed:

0.20

Space Group:

P 21 21 21

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Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA polymerase I
Gene (Uniprot):DPO1
Mutagens:F710Y
Chain IDs:A, B (auth: D)
Chain Length:580
Number of Molecules:2
Biological Source:Geobacillus stearothermophilus

UniProt ID:D9N168 EC:2.7.7.7

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(*CP*GP*AP*TP*CP*AP*
Chain IDs:C (auth: B), E
Chain Length:9
Number of Molecules:2
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(*CP*GP*(3DR)P*AP*CP
Chain IDs:D (auth: C), F
Chain Length:12
Number of Molecules:2
Biological Source:synthetic construct

Ligand Molecules