ISDA: 9WDX

Deposition Date 2025-08-19

Release Date 2026-08-26

Last Version Date 2026-08-26

Entry Detail

PDB ID:

9WDX

Keywords:

HYDROLASE

Title:

Structure of SpCas9 with engineered disulfide bonds (R1210C/V1280C and L1119C/P1128C)

Biological Source:

Source Organism(s):

Streptococcus pyogenes serotype M1 (Taxon ID: 301447)

Expression System(s):

Escherichia coli k-12

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

3.72 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

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Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:CRISPR-associated endonucleas
Gene (Uniprot):cas9
Mutagens:R1210C/V1280C/L1119C/P1128C
Chain IDs:A (auth: P)
Chain Length:1379
Number of Molecules:1
Biological Source:Streptococcus pyogenes serotype M1

UniProt ID:Q99ZW2 Pfam ID:PF16593, PF16592, PF13395, PF22702, PF16595 EC:3.1.-.-

Ligand Molecules

Primary Citation

Engineering Enhanced Thermostability and High-Temperature Activity in SpCas9 through Strategic C-Terminal Domain Disulfide Bond Incorporation

Teo, Z.T, Hartono, Y.D, Xue, B, Jia, X, Ravi, L.R.V, Qu, K, Carstens, C.-P, Bruhn, L, Yew, W.S.Show

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Primary Citation of related structures

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