ISDA: 9PI4

Deposition Date 2025-07-10

Release Date 2026-04-15

Last Version Date 2026-04-15

Entry Detail

PDB ID:

9PI4

Keywords:

DNA BINDING PROTEIN/DNA

Title:

Poly dT bound form of Single stranded DNA-binding protein(ICP8) from Herpes simplex virus-1. Mutations: C254S, C455S

Biological Source:

Source Organism(s):

Human alphaherpesvirus 1 (Taxon ID: 10298)
synthetic construct (Taxon ID: 32630)

Expression System(s):

Spodoptera frugiperda

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

3.10 Å

R-Value Free:

0.23

R-Value Work:

0.19

R-Value Observed:

0.19

Space Group:

P 32 2 1

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Major DNA-binding protein
Gene (Uniprot):DBP
Mutagens:C254S, C455S
Chain IDs:A
Chain Length:1128
Number of Molecules:1
Biological Source:Human alphaherpesvirus 1

UniProt ID:P04296 Pfam ID:PF00747

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(P*TP*TP*TP*TP*TP*TP
Chain IDs:B (auth: G)
Chain Length:8
Number of Molecules:1
Biological Source:synthetic construct

Ligand Molecules

Primary Citation

The crystal structure of the herpes virus ICP8 protein in complex with single-stranded DNA reveals the molecular determinants of nucleotide recognition.

Erlandsen, H, Krucinska, J, Wilderman, P.R, Makkay, A.M, Szczepaniak, R, Wright, L.R, Weller, S.K, Wright, D.L.Show

J. Biol. Chem. ? 111366 111366 (2026)

PMID: 41833734 DOI: 10.1016/j.jbc.2026.111366

Primary Citation of related structures

Feedback Form