9OYI image
Deposition Date 2025-06-04
Release Date 2026-04-29
Last Version Date 2026-04-29
Entry Detail
PDB ID:
9OYI
Keywords:
REPLICATION/DNA
Title:
Structure of the E. coli clamp loader DnaX-complex loading beta-clamp onto 10-nt gapped DNA in state 2 conformer 1 with fully open clamp and unsettled DNA
Biological Source:
Source Organism(s):
Escherichia coli (Taxon ID: 562)
DNA molecule (Taxon ID: 2853804)
Expression System(s):
Escherichia coli, Dna molecule
Method Details:
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
2.54 Å
Aggregation State:
PARTICLE
Reconstruction Method:
SINGLE PARTICLE
9OYI validation report missing
Macromolecular Entities
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:DNA polymerase III subunit de
Gene (Uniprot):holA
Chain IDs:A
Chain Length:343
Number of Molecules:1
Biological Source:Escherichia coli
UniProt ID:P28630 EC:3.6.4.B8
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:DNA polymerase III subunit ta
Gene (Uniprot):dnaX
Chain IDs:B, C, D
Chain Length:643
Number of Molecules:3
Biological Source:Escherichia coli
UniProt ID:P06710 EC:3.6.4.B8
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:DNA polymerase III subunit de
Gene (Uniprot):holB
Chain IDs:E
Chain Length:334
Number of Molecules:1
Biological Source:Escherichia coli
UniProt ID:P28631 EC:3.6.4.B8
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Beta sliding clamp
Chain IDs:F, G
Chain Length:366
Number of Molecules:2
Biological Source:Escherichia coli
UniProt ID:P0A990
Polymer Type:polydeoxyribonucleotide
Molecule:10-nt gapped DNA template str
Chain IDs:I (auth: H)
Chain Length:50
Number of Molecules:1
Biological Source:DNA molecule
Polymer Type:polydeoxyribonucleotide/polyribonucleotide hybrid
Molecule:10-nt gapped DNA primer stran
Chain IDs:J (auth: I)
Chain Length:20
Number of Molecules:1
Biological Source:DNA molecule
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:DNA polymerase III subunit ps
Gene (Uniprot):holD
Chain IDs:H (auth: J)
Chain Length:137
Number of Molecules:1
Biological Source:Escherichia coli
UniProt ID:P28632 EC:3.6.4.B8
Ligand Molecules
AGS
MG
ZN
Primary Citation
The E. coli DnaX clamp loader sharply bends DNA to load beta-clamp at nicks and small gaps.
Biorxiv ? ? ? (2026)
PMID: 41648351 DOI: 10.64898/2026.01.17.700081
Primary Citation of related structures
9OYI
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