ISDA: 9DYS

Deposition Date 2024-10-14

Release Date 2025-01-29

Last Version Date 2026-06-03

Entry Detail

PDB ID:

9DYS

Keywords:

HYDROLASE

Title:

X-ray Crystallographic Structure of the Poly(Hexamethylene Adipamide) (Nylon66) Hydrolase Nyl50 at Room Temperature bound to tetraethylene glycol

Biological Source:

Source Organism(s):

Alphaproteobacteria bacterium (Taxon ID: 1913988)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

1.85 Å

R-Value Free:

0.17

R-Value Work:

0.15

R-Value Observed:

0.15

Space Group:

P 2 21 21

Download Validation Report

Macromolecular Entities

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Poly(hexamethylene adipamide)
Gene (Uniprot):EPN97_01225
Chain IDs:A, B
Chain Length:305
Number of Molecules:2
Biological Source:Alphaproteobacteria bacterium

UniProt ID:A0A522DD61,A0ACD6BAK9 Pfam ID:PF03576

Ligand Molecules

EDO

PG4

Primary Citation

Identification and characterization of substrate- and product-selective nylon hydrolases

Drufva, E.E, Cahill, J.F, Saint-Vincent, P.M, Williams, A.N, Bocharova, V, Capra, N, Meilleur, F, Carper, D.L, Bourgery, C, Miyazaki, K, Yonemura, M, Shiraishi, Y, Parks, J.M, Zhou, M, Dishner, I.T, Foster, J.C, Koehler, S.J, Valentino, H.R, Sedova, A, Kertesz, V, Vasileva, D.P, Hochanadel, L.H, Figg, C.A, Negoro, S, Kato, D.I, Chen, S.H, Michener, J.K.Show

Chem Catal 5 101418 ? (2025)

DOI: 10.1016/j.checat.2025.101418

Primary Citation of related structures

Feedback Form