ISDA: 9AR7

Deposition Date 2024-02-22

Release Date 2025-02-26

Last Version Date 2026-04-15

Entry Detail

PDB ID:

9AR7

Keywords:

DNA BINDING PROTEIN/DNA/RNA

Title:

CryoEM structure of ThermoCas9 bound with target DNA strand only

Biological Source:

Source Organism(s):

Geobacillus thermodenitrificans (Taxon ID: 33940)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.52 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

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Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:CRISPR-associated endonucleas
Chain IDs:D (auth: A)
Chain Length:1082
Number of Molecules:1
Biological Source:Geobacillus thermodenitrificans

UniProt ID:A0A1W6VMQ3

Polymer Type:polyribonucleotide
Molecule:single guide RNA
Chain IDs:A (auth: B)
Chain Length:149
Number of Molecules:1
Biological Source:Geobacillus thermodenitrificans

Polymer Type:polydeoxyribonucleotide
Molecule:cleaved 3' target DNA strand
Chain IDs:B (auth: C)
Chain Length:40
Number of Molecules:1
Biological Source:Geobacillus thermodenitrificans

Polymer Type:polydeoxyribonucleotide
Molecule:cleaved 5' target strand
Chain IDs:C (auth: P)
Chain Length:14
Number of Molecules:1
Biological Source:Geobacillus thermodenitrificans

Ligand Molecules