ISDA: 9AR6

Deposition Date 2024-02-22

Release Date 2025-02-26

Last Version Date 2026-04-15

Entry Detail

PDB ID:

9AR6

Keywords:

DNA BINDING PROTEIN/DNA/RNA

Title:

CryoEM structure of ThermoCas9 in pre-cleavage state bound with the DNA containing NNNNCCA PAM

Biological Source:

Source Organism(s):

Geobacillus thermodenitrificans (Taxon ID: 33940)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.77 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:CRISPR-associated endonucleas
Chain IDs:A
Chain Length:1082
Number of Molecules:1
Biological Source:Geobacillus thermodenitrificans

UniProt ID:A0A1W6VMQ3

Polymer Type:polyribonucleotide
Molecule:single guide RNA
Chain IDs:D (auth: B)
Chain Length:147
Number of Molecules:1
Biological Source:Geobacillus thermodenitrificans

Polymer Type:polydeoxyribonucleotide
Molecule:non-target DNA
Chain IDs:B (auth: D)
Chain Length:12
Number of Molecules:1
Biological Source:Geobacillus thermodenitrificans

Polymer Type:polydeoxyribonucleotide
Molecule:target-strand DNA
Chain IDs:C (auth: P)
Chain Length:40
Number of Molecules:1
Biological Source:Geobacillus thermodenitrificans

Ligand Molecules

Primary Citation

Molecular basis for methylation-sensitive editing by Cas9

Roth, M.O, Shu, Y, Zhao, Y, Trasanidou, D, Hoffman, R.D, Trasanidis, N, Gelasco, M.K, Medina, M.L, Das, D, Rai, J, Wang, B, Li, H.Show

Nature ? ? ? (2026)

DOI: 10.1038/s41586-026-10384-z

Primary Citation of related structures

Feedback Form