ISDA: 9AR5

Deposition Date 2024-02-22

Release Date 2025-02-26

Last Version Date 2026-04-15

Entry Detail

PDB ID:

9AR5

Keywords:

DNA BINDING PROTEIN/DNA/RNA

Title:

Structure of Acidothermus cellulolyticus Cas9 bound with methylated DNA

Biological Source:

Source Organism(s):

Acidothermus cellulolyticus (Taxon ID: 28049)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.88 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:CRISPR-associated endonucleas
Gene (Uniprot):Acel_1951
Chain IDs:A
Chain Length:1138
Number of Molecules:1
Biological Source:Acidothermus cellulolyticus

UniProt ID:A0LWB3 Pfam ID:PF18470, PF01844, PF18541, PF17893, PF17894, PF18525

Polymer Type:polyribonucleotide
Molecule:RNA (92-MER)
Chain IDs:B
Chain Length:106
Number of Molecules:1
Biological Source:Acidothermus cellulolyticus

Polymer Type:polydeoxyribonucleotide
Molecule:DNA/RNA (5'-D(P*TP*AP*CP*A)-R
Chain IDs:D
Chain Length:11
Number of Molecules:1
Biological Source:Acidothermus cellulolyticus

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (25-MER)
Chain IDs:C (auth: X)
Chain Length:25
Number of Molecules:1
Biological Source:Acidothermus cellulolyticus

Ligand Molecules

Primary Citation

Molecular basis for methylation-sensitive editing by Cas9

Roth, M.O, Shu, Y, Zhao, Y, Trasanidou, D, Hoffman, R.D, Trasanidis, N, Gelasco, M.K, Medina, M.L, Das, D, Rai, J, Wang, B, Li, H.Show

Nature ? ? ? (2026)

DOI: 10.1038/s41586-026-10384-z

Primary Citation of related structures

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