ISDA: 9AR4

Deposition Date 2024-02-22

Release Date 2025-02-26

Last Version Date 2026-04-15

Entry Detail

PDB ID:

9AR4

Keywords:

DNA BINDING PROTEIN/DNA/RNA

Title:

CryoEM structure of ThermoCas9 in post-cleavage state bound with the DNA containing NNNNCCA PAM

Biological Source:

Source Organism(s):

Geobacillus thermodenitrificans (Taxon ID: 33940)
synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.20 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:CRISPR-associated endonucleas
Chain IDs:F (auth: A)
Chain Length:1082
Number of Molecules:1
Biological Source:Geobacillus thermodenitrificans

UniProt ID:A0A1W6VMQ3

Polymer Type:polyribonucleotide
Molecule:Single guide RNA
Chain IDs:A (auth: B)
Chain Length:149
Number of Molecules:1
Biological Source:Geobacillus thermodenitrificans

Polymer Type:polydeoxyribonucleotide
Molecule:Cleaved 3' target DNA strand
Chain IDs:B (auth: C)
Chain Length:26
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:Cleaved 3' non-target DNA str
Chain IDs:C (auth: D)
Chain Length:14
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:Cleaved 5' target DNA strand
Chain IDs:D (auth: P)
Chain Length:14
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:Cleaved 5' non-target DNA str
Chain IDs:E (auth: X)
Chain Length:5
Number of Molecules:1
Biological Source:synthetic construct

Ligand Molecules