ISDA: 5M0T

Deposition Date 2016-10-05

Release Date 2016-12-07

Last Version Date 2024-01-17

Entry Detail

PDB ID:

5M0T

Keywords:

OXIDOREDUCTASE

Title:

Alpha-ketoglutarate-dependent non-heme iron oxygenase EasH

Biological Source:

Source Organism(s):

Aspergillus japonicus (Taxon ID: 34381)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

2.20 Å

R-Value Free:

0.24

R-Value Work:

0.21

R-Value Observed:

0.21

Space Group:

P 21 21 21

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Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:Alpha-ketoglutarate-dependent
Chain IDs:A, B
Chain Length:294
Number of Molecules:2
Biological Source:Aspergillus japonicus

UniProt ID:A0A1L1QK38 Pfam ID:PF05721

Ligand Molecules

AKG

FE2

Primary Citation

Structural characterization of EasH (Aspergillus japonicus) - an oxidase involved in cycloclavine biosynthesis.

Jakubczyk, D, Caputi, L, Stevenson, C.E, Lawson, D.M, O'Connor, S.E.Show

Chem. Commun. (Camb.) 52 14306 14309 (2016)

PMID: 27885368 DOI: 10.1039/c6cc08438a

Abstact

Aj_EasH is a non-heme iron- and α-keto-glutarate-dependent oxidase that is responsible for an unusual cyclopropyl ring formation in the biosynthesis of the fungal ergot alkaloid cycloclavine. The three dimensional structure of Aj_EasH (2.2 Å resolution) reported here provides insight into the mechanism of this unusual and complex reaction.

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Primary Citation of related structures

Abstact

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