5LUF image
Deposition Date 2016-09-08
Release Date 2016-11-30
Last Version Date 2024-11-13
Entry Detail
PDB ID:
5LUF
Keywords:
OXIDOREDUCTASE
Title:
Cryo-EM of bovine respirasome
Biological Source:
Source Organism(s):
Bos taurus (Taxon ID: 9913)
Method Details:
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
9.10 Å
Aggregation State:
PARTICLE
Reconstruction Method:
SINGLE PARTICLE
5LUF validation report missing
Macromolecular Entities
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome c oxidase subunit
Gene (Uniprot):COX8B
Chain IDs:IA (auth: 0)
Chain Length:46
Number of Molecules:1
Biological Source:Bos taurus
UniProt ID:P10175 Pfam ID:PF02285 EC:7.1.1.9
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome c oxidase subunit
Gene (Uniprot):COX4I1
Chain IDs:Z (auth: 1)
Chain Length:147
Number of Molecules:1
Biological Source:Bos taurus
UniProt ID:P00423 Pfam ID:PF02936 EC:7.1.1.9
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome c oxidase subunit
Gene (Uniprot):COX5A
Chain IDs:AA (auth: 2)
Chain Length:109
Number of Molecules:1
Biological Source:Bos taurus
UniProt ID:P00426 Pfam ID:PF02284 EC:7.1.1.9
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome c oxidase subunit
Gene (Uniprot):COX5B
Chain IDs:BA (auth: 3)
Chain Length:98
Number of Molecules:1
Biological Source:Bos taurus
UniProt ID:P00428 Pfam ID:PF01215 EC:7.1.1.9
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome c oxidase subunit
Gene (Uniprot):COX6A2
Chain IDs:CA (auth: 4)
Chain Length:84
Number of Molecules:1
Biological Source:Bos taurus
UniProt ID:P07471 Pfam ID:PF02046 EC:7.1.1.9
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome c oxidase subunit
Gene (Uniprot):COX6B1
Chain IDs:DA (auth: 5)
Chain Length:85
Number of Molecules:1
Biological Source:Bos taurus
UniProt ID:P00429 Pfam ID:PF02297 EC:7.1.1.9
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome c oxidase subunit
Gene (Uniprot):COX6C
Chain IDs:EA (auth: 6)
Chain Length:73
Number of Molecules:1
Biological Source:Bos taurus
UniProt ID:P04038 Pfam ID:PF02937 EC:7.1.1.9
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome c oxidase subunit
Gene (Uniprot):COX7A1
Chain IDs:FA (auth: 7)
Chain Length:59
Number of Molecules:1
Biological Source:Bos taurus
UniProt ID:P07470 Pfam ID:PF02238 EC:7.1.1.9
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome c oxidase subunit
Gene (Uniprot):COX7B
Chain IDs:GA (auth: 8)
Chain Length:56
Number of Molecules:1
Biological Source:Bos taurus
UniProt ID:P13183 Pfam ID:PF05392 EC:7.1.1.9
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome c oxidase subunit
Gene (Uniprot):COX7C
Chain IDs:HA (auth: 9)
Chain Length:47
Number of Molecules:1
Biological Source:Bos taurus
UniProt ID:P00430 Pfam ID:PF02935 EC:7.1.1.9
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:JA (auth: A)
Chain Length:111
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:KA (auth: B)
Chain Length:379
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:LA (auth: C)
Chain Length:446
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:MA (auth: D)
Chain Length:241
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:NA (auth: E)
Chain Length:196
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:OA (auth: F)
Chain Length:411
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:PA (auth: G)
Chain Length:538
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:QA (auth: H)
Chain Length:78
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:RA (auth: I)
Chain Length:78
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:SA (auth: J)
Chain Length:171
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:TA (auth: K)
Chain Length:56
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:UA (auth: L)
Chain Length:446
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:VA (auth: M)
Chain Length:453
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:WA (auth: N)
Chain Length:345
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:XA (auth: O)
Chain Length:379
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:YA (auth: P)
Chain Length:303
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:ZA (auth: Q)
Chain Length:196
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:AB (auth: R)
Chain Length:47
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:BB (auth: S)
Chain Length:81
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:CB (auth: T)
Chain Length:78
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:IB (auth: U)
Chain Length:78
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:DB (auth: V)
Chain Length:62
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:EB (auth: W)
Chain Length:72
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:FB (auth: X)
Chain Length:330
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:GB (auth: Y)
Chain Length:227
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:JB (auth: Z)
Chain Length:261
Number of Molecules:1
Biological Source:Bos taurus
Protein Blast
Polymer Type:polypeptide(L)
Molecule:complex I
Chain IDs:HB (auth: a)
Chain Length:111
Number of Molecules:1
Biological Source:Bos taurus
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome b
Gene (Uniprot):MT-CYB
Chain IDs:C (auth: b), N (auth: o)
Chain Length:379
Number of Molecules:2
Biological Source:Bos taurus
UniProt ID:P00157 Pfam ID:PF00033, PF00032
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome b-c1 complex subun
Gene (Uniprot):UQCRC1
Chain IDs:A (auth: c), L (auth: l)
Chain Length:446
Number of Molecules:2
Biological Source:Bos taurus
UniProt ID:P31800 Pfam ID:PF00675, PF05193 EC:7.1.1.8
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome c1, heme protein,
Gene (Uniprot):CYC1
Chain IDs:D (auth: d), O (auth: p)
Chain Length:241
Number of Molecules:2
Biological Source:Bos taurus
UniProt ID:P00125 Pfam ID:PF02167
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome b-c1 complex subun
Gene (Uniprot):UQCRFS1
Chain IDs:E (auth: e), P (auth: q)
Chain Length:196
Number of Molecules:2
Biological Source:Bos taurus
UniProt ID:P13272 Pfam ID:PF02921, PF00355, PF09165 EC:1.10.2.2
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome b-c1 complex subun
Gene (Uniprot):UQCRB
Chain IDs:F (auth: f), Q (auth: r)
Chain Length:411
Number of Molecules:2
Biological Source:Bos taurus
UniProt ID:P00129 Pfam ID:PF02271
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome b-c1 complex subun
Gene (Uniprot):UQCRQ
Chain IDs:G (auth: g), R (auth: s)
Chain Length:81
Number of Molecules:2
Biological Source:Bos taurus
UniProt ID:P13271 Pfam ID:PF02939
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome b-c1 complex subun
Gene (Uniprot):UQCRH
Chain IDs:H (auth: h), S (auth: t)
Chain Length:78
Number of Molecules:2
Biological Source:Bos taurus
UniProt ID:P00126 Pfam ID:PF02320
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome b-c1 complex subun
Gene (Uniprot):UQCRFS1
Chain IDs:I (auth: i), T (auth: u)
Chain Length:78
Number of Molecules:2
Biological Source:Bos taurus
UniProt ID:P13272 Pfam ID:PF02921, PF00355, PF09165 EC:1.10.2.2
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome b-c1 complex subun
Gene (Uniprot):UQCR10
Chain IDs:J (auth: j), U (auth: v)
Chain Length:62
Number of Molecules:2
Biological Source:Bos taurus
UniProt ID:P00130 Pfam ID:PF05365
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome b-c1 complex subun
Gene (Uniprot):UQCR11
Chain IDs:K (auth: k), V (auth: w)
Chain Length:72
Number of Molecules:2
Biological Source:Bos taurus
UniProt ID:P07552 Pfam ID:PF08997
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome b-c1 complex subun
Gene (Uniprot):UQCRC2
Chain IDs:B (auth: m), M (auth: n)
Chain Length:345
Number of Molecules:2
Biological Source:Bos taurus
UniProt ID:P23004 Pfam ID:PF00675, PF05193
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome c oxidase subunit
Gene (Uniprot):MT-CO1
Chain IDs:W (auth: x)
Chain Length:330
Number of Molecules:1
Biological Source:Bos taurus
UniProt ID:P00396 Pfam ID:PF00115 EC:7.1.1.9
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome c oxidase subunit
Gene (Uniprot):MT-CO2
Chain IDs:X (auth: y)
Chain Length:227
Number of Molecules:1
Biological Source:Bos taurus
UniProt ID:P68530 Pfam ID:PF02790, PF00116
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cytochrome c oxidase subunit
Gene (Uniprot):MT-CO3
Chain IDs:Y (auth: z)
Chain Length:261
Number of Molecules:1
Biological Source:Bos taurus
UniProt ID:P00415 Pfam ID:PF00510 EC:7.1.1.9
Ligand Molecules
CU
FES
HEA
HEC
HEM
MG
SF4
ZN
Primary Citation
Functional asymmetry and electron flow in the bovine respirasome.
Elife 5 ? ? (2016)
PMID: 27830641 DOI: 10.7554/eLife.21290

Abstact

Respirasomes are macromolecular assemblies of the respiratory chain complexes I, III and IV in the inner mitochondrial membrane. We determined the structure of supercomplex I1III2IV1 from bovine heart mitochondria by cryo-EM at 9 Å resolution. Most protein-protein contacts between complex I, III and IV in the membrane are mediated by supernumerary subunits. Of the two Rieske iron-sulfur cluster domains in the complex III dimer, one is resolved, indicating that this domain is immobile and unable to transfer electrons. The central position of the active complex III monomer between complex I and IV in the respirasome is optimal for accepting reduced quinone from complex I over a short diffusion distance of 11 nm, and delivering reduced cytochrome c to complex IV. The functional asymmetry of complex III provides strong evidence for directed electron flow from complex I to complex IV through the active complex III monomer in the mammalian supercomplex.

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Protein

Chemical

Disease

Primary Citation of related structures
5LUF
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