ISDA: 5CIZ

Deposition Date 2015-07-13

Release Date 2016-06-29

Last Version Date 2023-09-27

Entry Detail

PDB ID:

5CIZ

Keywords:

Gene regulation/DNA

Title:

E. coli RNA polymerase alpha subunit CTD in complex with CAP and DNA: A(5)-tract binding site for alpha CTD

Biological Source:

Source Organism(s):

Escherichia coli (strain K12) (Taxon ID: 83333)
Escherichia coli (Taxon ID: 562)

Expression System(s):

Escherichia coli bl21(de3)

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

5.01 Å

R-Value Free:

0.21

R-Value Work:

0.17

R-Value Observed:

0.18

Space Group:

P 62 2 2

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:cAMP-activated global transcr
Gene (Uniprot):crp
Chain IDs:A
Chain Length:209
Number of Molecules:1
Biological Source:Escherichia coli (strain K12)

UniProt ID:P0ACJ8 Pfam ID:PF00027, PF13545

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoA
Chain IDs:B
Chain Length:84
Number of Molecules:1
Biological Source:Escherichia coli (strain K12)

UniProt ID:P0A7Z4 Pfam ID:PF03118 EC:2.7.7.6

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(*CP*TP*TP*TP*TP*TP*
Chain IDs:C (auth: D)
Chain Length:20
Number of Molecules:1
Biological Source:Escherichia coli

Polymer Type:polydeoxyribonucleotide
Molecule:DNA (5'-D(*CP*TP*AP*GP*AP*TP*
Chain IDs:D (auth: E)
Chain Length:24
Number of Molecules:1
Biological Source:Escherichia coli

Ligand Molecules

CMP

Primary Citation

The RNA Polymerase alpha Subunit Recognizes the DNA Shape of the Upstream Promoter Element

Lara-Gonzalez, S, Machado, A.C.D, Rao, S, Napoli, A.A, Birktoft, J, Di Felice, R, Rohs, R, Lawson, C.L.Show

Biochemistry ? ? ? (2020)

DOI: 10.1021/acs.biochem.0c00571

Primary Citation of related structures

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