3CO8 image
Deposition Date 2008-03-27
Release Date 2008-04-08
Last Version Date 2025-03-26
Entry Detail
PDB ID:
3CO8
Keywords:
ISOMERASE
Title:
Crystal structure of alanine racemase from Oenococcus oeni
Biological Source:
Source Organism(s):
Oenococcus oeni (Taxon ID: 203123)
Expression System(s):
Escherichia coli bl21(de3)
Method Details:
Experimental Method:
X-RAY DIFFRACTION
Resolution:
1.70 Å
R-Value Free:
0.25
R-Value Work:
0.24
R-Value Observed:
0.24
Space Group:
P 1 21 1
3CO8 validation report missing
Macromolecular Entities
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Alanine racemase
Gene (Uniprot):OEOE_0162
Chain IDs:A, B
Chain Length:380
Number of Molecules:2
Biological Source:Oenococcus oeni
UniProt ID:Q04HB7 Pfam ID:PF01168, PF00842 EC:5.1.1.5
Modified Residue
Compound ID Chain ID Parent Comp ID Details 2D Image
MSE A MET SELENOMETHIONINE
Ligand Molecules
PLP
Primary Citation
Structure of alanine racemase from Oenococcus oeni with bound pyridoxal 5'-phosphate.
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 69 15 19 (2013)
PMID: 23295479 DOI: 10.1107/S1744309112047276

Abstact

The crystal structure of alanine racemase from Oenococcus oeni has been determined at 1.7 Å resolution using the single-wavelength anomalous dispersion (SAD) method and selenium-labelled protein. The protein exists as a symmetric dimer in the crystal, with both protomers contributing to the two active sites. Pyridoxal 5'-phosphate, a cofactor, is bound to each monomer and forms a Schiff base with Lys39. Structural comparison of alanine racemase from O. oeni (Alr) with homologous family members revealed similar domain organization and cofactor binding.

Legend

Protein

Chemical

Disease

Primary Citation of related structures
3CO8
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