ISDA: 2MW6

Deposition Date 2014-10-28

Release Date 2015-07-01

Last Version Date 2026-08-12

Entry Detail

PDB ID:

2MW6

Keywords:

TOXIN

Title:

Structure of the bee venom toxin melittin with [(C5H5)Ru]+ fragment attached to the tryptophan residue

Biological Source:

Source Organism(s):

Apis mellifera (Taxon ID: 7460)

Method Details:

Experimental Method:

SOLUTION NMR

Conformers Calculated:

Conformers Submitted:

Selection Criteria:

all calculated structures submitted

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Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Melittin
Gene (Uniprot):MELT
Chain IDs:A
Chain Length:27
Number of Molecules:1
Biological Source:Apis mellifera

UniProt ID:P01501 Pfam ID:PF01372

Ligand Molecules

3UQ

Primary Citation

Selective ruthenium labeling of the tryptophan residue in the bee venom Peptide melittin.

Perekalin, D.S, Novikov, V.V, Pavlov, A.A, Ivanov, I.A, Anisimova, N.Y, Kopylov, A.N, Volkov, D.S, Seregina, I.F, Bolshov, M.A, Kudinov, A.R.Show

Chemistry 21 4923 4925 (2015)

PMID: 25688543 DOI: 10.1002/chem.201406510

Abstact

Melittin is a membrane-active peptide from bee venom with promising antimicrobial and anticancer activity. Herein we report on a simple and selective method for labeling of the tryptophan residue in melittin by the organometallic fragment [(C5 H5)Ru](+) in aqueous solution and in air. Ruthenium coordination does not disturb the secondary structure of the peptide (as verified by 2D NMR spectroscopy), but changes the pattern of its intermolecular interactions resulting in an 11-fold decrease of hemolytic activity. The high stability of the organometallic conjugate allowed the establishment of the biodistribution of the labeled melittin in mice by inductively coupled plasma MS analysis of ruthenium.

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Protein

Chemical

Disease

Primary Citation of related structures

Abstact

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