28RO image
Deposition Date 2026-02-16
Release Date 2026-04-29
Last Version Date 2026-06-10
Entry Detail
PDB ID:
28RO
Keywords:
VIRUS LIKE PARTICLE
Title:
split variant of Aquifex aeolicus lumazine synthase-derived nucleocapsid variant NC-4
Biological Source:
Source Organism(s):
Aquifex aeolicus (Taxon ID: 63363)
Expression System(s):
Escherichia coli bl21(de3)
Method Details:
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
3.10 Å
Aggregation State:
PARTICLE
Reconstruction Method:
SINGLE PARTICLE
28RO validation report missing
Macromolecular Entities
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:6,7-dimethyl-8-ribityllumazin
Gene (Uniprot):ribH
Chain IDs:A (auth: 0A), B (auth: 0B), C (auth: 0C), D (auth: 0D), I (auth: 1A), J (auth: 1B), K (auth: 1C), L (auth: 1D), Q (auth: 2A), R (auth: 2B), S (auth: 2C), T (auth: 2D), Y (auth: 3A), Z (auth: 3B), AA (auth: 3C), BA (auth: 3D), GA (auth: 4A), HA (auth: 4B), IA (auth: 4C), JA (auth: 4D), OA (auth: 5A), PA (auth: 5B), QA (auth: 5C), RA (auth: 5D), WA (auth: 6A), XA (auth: 6B), YA (auth: 6C), ZA (auth: 6D), EB (auth: 7A), FB (auth: 7B), GB (auth: 7C), HB (auth: 7D), MB (auth: AA), NB (auth: AB), OB (auth: AC), PB (auth: AD), UB (auth: BA), VB (auth: BB), WB (auth: BC), XB (auth: BD), CC (auth: CA), DC (auth: CB), EC (auth: CC), FC (auth: CD), KC (auth: DA), LC (auth: DB), MC (auth: DC), NC (auth: DD), SC (auth: EA), TC (auth: EB), UC (auth: EC), VC (auth: ED), AD (auth: FA), BD (auth: FB), CD (auth: FC), DD (auth: FD), ID (auth: GA), JD (auth: GB), KD (auth: GC), LD (auth: GD), QD (auth: HA), RD (auth: HB), SD (auth: HC), TD (auth: HD), YD (auth: IA), ZD (auth: IB), AE (auth: IC), BE (auth: ID), GE (auth: JA), HE (auth: JB), IE (auth: JC), JE (auth: JD), OE (auth: KA), PE (auth: KB), QE (auth: KC), RE (auth: KD), WE (auth: LA), XE (auth: LB), YE (auth: LC), ZE (auth: LD), EF (auth: MA), FF (auth: MB), GF (auth: MC), HF (auth: MD), MF (auth: NA), NF (auth: NB), OF (auth: NC), PF (auth: ND), UF (auth: OA), VF (auth: OB), WF (auth: OC), XF (auth: OD), CG (auth: PA), DG (auth: PB), EG (auth: PC), FG (auth: PD), KG (auth: QA), LG (auth: QB), MG (auth: QC), NG (auth: QD), SG (auth: RA), TG (auth: RB), UG (auth: RC), VG (auth: RD), AH (auth: SA), BH (auth: SB), CH (auth: SC), DH (auth: SD), IH (auth: TA), JH (auth: TB), KH (auth: TC), LH (auth: TD), QH (auth: UA), RH (auth: UB), SH (auth: UC), TH (auth: UD), YH (auth: VA), ZH (auth: VB), AI (auth: VC), BI (auth: VD), GI (auth: WA), HI (auth: WB), II (auth: WC), JI (auth: WD), OI (auth: XA), PI (auth: XB), QI (auth: XC), RI (auth: XD), WI (auth: YA), XI (auth: YB), YI (auth: YC), ZI (auth: YD), EJ (auth: ZA), FJ (auth: ZB), GJ (auth: ZC), HJ (auth: ZD), MJ (auth: aA), NJ (auth: aB), OJ (auth: aC), PJ (auth: aD), UJ (auth: bA), VJ (auth: bB), WJ (auth: bC), XJ (auth: bD), CK (auth: cA), DK (auth: cB), EK (auth: cC), FK (auth: cD), KK (auth: dA), LK (auth: dB), MK (auth: dC), NK (auth: dD), SK (auth: eA), TK (auth: eB), UK (auth: eC), VK (auth: eD), AL (auth: fA), BL (auth: fB), CL (auth: fC), DL (auth: fD), IL (auth: gA), JL (auth: gB), KL (auth: gC), LL (auth: gD), QL (auth: hA), RL (auth: hB), SL (auth: hC), TL (auth: hD), YL (auth: iA), ZL (auth: iB), AM (auth: iC), BM (auth: iD), GM (auth: jA), HM (auth: jB), IM (auth: jC), JM (auth: jD), OM (auth: kA), PM (auth: kB), QM (auth: kC), RM (auth: kD), WM (auth: lA), XM (auth: lB), YM (auth: lC), ZM (auth: lD), EN (auth: mA), FN (auth: mB), GN (auth: mC), HN (auth: mD), MN (auth: nA), NN (auth: nB), ON (auth: nC), PN (auth: nD), UN (auth: oA), VN (auth: oB), WN (auth: oC), XN (auth: oD), CO (auth: pA), DO (auth: pB), EO (auth: pC), FO (auth: pD), KO (auth: qA), LO (auth: qB), MO (auth: qC), NO (auth: qD), SO (auth: rA), TO (auth: rB), UO (auth: rC), VO (auth: rD), AP (auth: sA), BP (auth: sB), CP (auth: sC), DP (auth: sD), IP (auth: tA), JP (auth: tB), KP (auth: tC), LP (auth: tD), QP (auth: uA), RP (auth: uB), SP (auth: uC), TP (auth: uD), YP (auth: vA), ZP (auth: vB), AQ (auth: vC), BQ (auth: vD), GQ (auth: wA), HQ (auth: wB), IQ (auth: wC), JQ (auth: wD), OQ (auth: xA), PQ (auth: xB), QQ (auth: xC), RQ (auth: xD), WQ (auth: yA), XQ (auth: yB), YQ (auth: yC), ZQ (auth: yD), ER (auth: zA), FR (auth: zB), GR (auth: zC), HR (auth: zD)
Chain Length:86
Number of Molecules:240
Biological Source:Aquifex aeolicus
UniProt ID:O66529 Pfam ID:PF00885 EC:2.5.1.78
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:6,7-dimethyl-8-ribityllumazin
Gene (Uniprot):ribH
Chain IDs:E (auth: 0a), F (auth: 0b), G (auth: 0c), H (auth: 0d), M (auth: 1a), N (auth: 1b), O (auth: 1c), P (auth: 1d), U (auth: 2a), V (auth: 2b), W (auth: 2c), X (auth: 2d), CA (auth: 3a), DA (auth: 3b), EA (auth: 3c), FA (auth: 3d), KA (auth: 4a), LA (auth: 4b), MA (auth: 4c), NA (auth: 4d), SA (auth: 5a), TA (auth: 5b), UA (auth: 5c), VA (auth: 5d), AB (auth: 6a), BB (auth: 6b), CB (auth: 6c), DB (auth: 6d), IB (auth: 7a), JB (auth: 7b), KB (auth: 7c), LB (auth: 7d), QB (auth: Aa), RB (auth: Ab), SB (auth: Ac), TB (auth: Ad), YB (auth: Ba), ZB (auth: Bb), AC (auth: Bc), BC (auth: Bd), GC (auth: Ca), HC (auth: Cb), IC (auth: Cc), JC (auth: Cd), OC (auth: Da), PC (auth: Db), QC (auth: Dc), RC (auth: Dd), WC (auth: Ea), XC (auth: Eb), YC (auth: Ec), ZC (auth: Ed), ED (auth: Fa), FD (auth: Fb), GD (auth: Fc), HD (auth: Fd), MD (auth: Ga), ND (auth: Gb), OD (auth: Gc), PD (auth: Gd), UD (auth: Ha), VD (auth: Hb), WD (auth: Hc), XD (auth: Hd), CE (auth: Ia), DE (auth: Ib), EE (auth: Ic), FE (auth: Id), KE (auth: Ja), LE (auth: Jb), ME (auth: Jc), NE (auth: Jd), SE (auth: Ka), TE (auth: Kb), UE (auth: Kc), VE (auth: Kd), AF (auth: La), BF (auth: Lb), CF (auth: Lc), DF (auth: Ld), IF (auth: Ma), JF (auth: Mb), KF (auth: Mc), LF (auth: Md), QF (auth: Na), RF (auth: Nb), SF (auth: Nc), TF (auth: Nd), YF (auth: Oa), ZF (auth: Ob), AG (auth: Oc), BG (auth: Od), GG (auth: Pa), HG (auth: Pb), IG (auth: Pc), JG (auth: Pd), OG (auth: Qa), PG (auth: Qb), QG (auth: Qc), RG (auth: Qd), WG (auth: Ra), XG (auth: Rb), YG (auth: Rc), ZG (auth: Rd), EH (auth: Sa), FH (auth: Sb), GH (auth: Sc), HH (auth: Sd), MH (auth: Ta), NH (auth: Tb), OH (auth: Tc), PH (auth: Td), UH (auth: Ua), VH (auth: Ub), WH (auth: Uc), XH (auth: Ud), CI (auth: Va), DI (auth: Vb), EI (auth: Vc), FI (auth: Vd), KI (auth: Wa), LI (auth: Wb), MI (auth: Wc), NI (auth: Wd), SI (auth: Xa), TI (auth: Xb), UI (auth: Xc), VI (auth: Xd), AJ (auth: Ya), BJ (auth: Yb), CJ (auth: Yc), DJ (auth: Yd), IJ (auth: Za), JJ (auth: Zb), KJ (auth: Zc), LJ (auth: Zd), QJ (auth: aa), RJ (auth: ab), SJ (auth: ac), TJ (auth: ad), YJ (auth: ba), ZJ (auth: bb), AK (auth: bc), BK (auth: bd), GK (auth: ca), HK (auth: cb), IK (auth: cc), JK (auth: cd), OK (auth: da), PK (auth: db), QK (auth: dc), RK (auth: dd), WK (auth: ea), XK (auth: eb), YK (auth: ec), ZK (auth: ed), EL (auth: fa), FL (auth: fb), GL (auth: fc), HL (auth: fd), ML (auth: ga), NL (auth: gb), OL (auth: gc), PL (auth: gd), UL (auth: ha), VL (auth: hb), WL (auth: hc), XL (auth: hd), CM (auth: ia), DM (auth: ib), EM (auth: ic), FM (auth: id), KM (auth: ja), LM (auth: jb), MM (auth: jc), NM (auth: jd), SM (auth: ka), TM (auth: kb), UM (auth: kc), VM (auth: kd), AN (auth: la), BN (auth: lb), CN (auth: lc), DN (auth: ld), IN (auth: ma), JN (auth: mb), KN (auth: mc), LN (auth: md), QN (auth: na), RN (auth: nb), SN (auth: nc), TN (auth: nd), YN (auth: oa), ZN (auth: ob), AO (auth: oc), BO (auth: od), GO (auth: pa), HO (auth: pb), IO (auth: pc), JO (auth: pd), OO (auth: qa), PO (auth: qb), QO (auth: qc), RO (auth: qd), WO (auth: ra), XO (auth: rb), YO (auth: rc), ZO (auth: rd), EP (auth: sa), FP (auth: sb), GP (auth: sc), HP (auth: sd), MP (auth: ta), NP (auth: tb), OP (auth: tc), PP (auth: td), UP (auth: ua), VP (auth: ub), WP (auth: uc), XP (auth: ud), CQ (auth: va), DQ (auth: vb), EQ (auth: vc), FQ (auth: vd), KQ (auth: wa), LQ (auth: wb), MQ (auth: wc), NQ (auth: wd), SQ (auth: xa), TQ (auth: xb), UQ (auth: xc), VQ (auth: xd), AR (auth: ya), BR (auth: yb), CR (auth: yc), DR (auth: yd), IR (auth: za), JR (auth: zb), KR (auth: zc), LR (auth: zd)
Chain Length:86
Number of Molecules:240
Biological Source:Aquifex aeolicus
UniProt ID:O66529 Pfam ID:PF00885 EC:2.5.1.78
Ligand Molecules
NA
Primary Citation
An engineered closed-shell, two-component, 480-subunit nucleocapsid.
Proc.Natl.Acad.Sci.USA 123 e2530090123 e2530090123 (2026)
PMID: 42201976 DOI: 10.1073/pnas.2530090123

Abstact

Self-assembling protein cages are valuable nanoscale containers for biotechnology and medical applications. Two-component systems are especially attractive due to their potential for functional complexity. In this study, we demonstrate that the subunits of the 240-subunit nucleocapsid NC-4, which was previously evolved to package and protect its encoding mRNA, can be split into two fragments without disrupting cage assembly or structure, generating a two-component, 480-subunit capsid. This modification introduces additional termini on the cage's exterior surface, creating opportunities for functionalization. We exploited these new sites by genetically appending peptide and protein tags to the exterior surface of split NC-4 (spNC-4), enabling site-specific glycosylation via posttranslational modification and cell-specific delivery by targeted antibody recruitment. Our findings broaden the utility of the NC-4 nucleocapsid. By extension, splitting related protein compartments that bind diverse cargoes could offer a robust platform for biotechnological applications requiring simultaneous encapsulation and customizable surface modification.

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Disease

Primary Citation of related structures
28RO
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