ISDA: 24MR

Deposition Date 2026-03-11

Release Date 2026-07-22

Last Version Date 2026-07-22

Entry Detail

PDB ID:

24MR

Keywords:

HYDROLASE

Title:

Structure of the GH136 lacto-N-biosidase LnbX D418S glycosynthase mutant

Biological Source:

Source Organism(s):

Bifidobacterium longum (Taxon ID: 216816)

Expression System(s):

Escherichia coli bl21

Method Details:

Experimental Method:

X-RAY DIFFRACTION

Resolution:

2.47 Å

R-Value Free:

0.27

R-Value Work:

0.21

R-Value Observed:

0.21

Space Group:

P 21 21 21

Download Validation Report

Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:Lacto-N-biosidase
Gene (Uniprot):lnbX
Mutagens:D418S
Chain IDs:A, B
Chain Length:589
Number of Molecules:2
Biological Source:Bifidobacterium longum

UniProt ID:A0A024QYS6 EC:3.2.1.140

Ligand Molecules

Peptide-like Molecules

PRD_002584

Primary Citation

One-pot synthesis of lacto-N-tetraose using a glycosynthase engineered from GH136 lacto-N-biosidase LnbX.

Fujio, N, Umekage, R, Ishikawa, A, Honda, Y, Sugiyama, Y, Kitaoka, M, Katayama, T, Fushinobu, S, Yamada, C.Show

Glycobiology ? ? ? (2026)

PMID: 42429352 DOI: 10.1093/glycob/cwag054

Primary Citation of related structures

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