21EM image
Deposition Date 2025-12-10
Release Date 2026-06-17
Last Version Date 2026-06-17
Entry Detail
PDB ID:
21EM
Keywords:
METAL BINDING PROTEIN
Title:
Crystal structure of a cupin protein (tm1459, D50N/H52G/H92A/F104W mutant) soaked in CuSO4
Biological Source:
Source Organism(s):
Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (Taxon ID: 243274)
Expression System(s):
Escherichia coli bl21(de3)
Method Details:
Experimental Method:
X-RAY DIFFRACTION
Resolution:
1.60 Å
R-Value Free:
0.26
R-Value Work:
0.18
R-Value Observed:
0.19
Space Group:
P 21 21 21
21EM validation report missing
Macromolecular Entities
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:Cupin type-2 domain-containin
Gene (Uniprot):Tmari_1465
Mutagens:D50N/H52G/H92A/F104W
Chain IDs:A, B
Chain Length:118
Number of Molecules:2
Biological Source:Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
UniProt ID:Q9X1H0 Pfam ID:PF07883
Modified Residue
Compound ID Chain ID Parent Comp ID Details 2D Image
CSD A CYS modified residue
Ligand Molecules
6PC
CU
Primary Citation
Structure-Guided Surface Engineering of an Artificial Metallolyase for Highly Enantioselective Michael Addition
Chemcatchem 18 ? ? (2026)
DOI: 10.1002/cctc.70836
Primary Citation of related structures
21EM
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