1DPI image
Deposition Date 1987-08-11
Release Date 1987-10-16
Last Version Date 2024-02-07
Entry Detail
PDB ID:
1DPI
Keywords:
NUCLEOTIDYLTRANSFERASE
Title:
STRUCTURE OF LARGE FRAGMENT OF ESCHERICHIA COLI DNA POLYMERASE I COMPLEXED WITH D/TMP
Biological Source:
Source Organism(s):
Escherichia coli (Taxon ID: 562)
Method Details:
Experimental Method:
X-RAY DIFFRACTION
Resolution:
2.80 Å
R-Value Observed:
0.20
Space Group:
P 43
1DPI validation report missing
Macromolecular Entities
Structures with similar UniProt ID
Protein Blast
Polymer Type:polypeptide(L)
Molecule:DNA POLYMERASE I KLENOW FRAGM
Gene (Uniprot):polA
Chain IDs:A
Chain Length:605
Number of Molecules:1
Biological Source:Escherichia coli
UniProt ID:P00582 Pfam ID:PF01612, PF00476 EC:2.7.7.7
Ligand Molecules
ZN
Primary Citation
Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP.
Nature 313 762 766 (1985)
PMID: 3883192 DOI: 10.1038/313762a0

Abstact

The 3.3-A resolution crystal structure of the large proteolytic fragment of Escherichia coli DNA polymerase I complexed with deoxythymidine monophosphate consists of two domains, the smaller of which binds zinc-deoxythymidine monophosphate. The most striking feature of the larger domain is a deep crevice of the appropriate size and shape for binding double-stranded B-DNA. A flexible subdomain may allow the enzyme to surround completely the DNA substrate, thereby allowing processive nucleotide polymerization without enzyme dissociation.

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Primary Citation of related structures
1DPI
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