ISDA: 12CF

Deposition Date 2026-03-26

Release Date 2026-11-11

Last Version Date 2026-11-11

Entry Detail

PDB ID:

12CF

Keywords:

TRANSCRIPTION

Title:

Head-on RNAP-RNAP Collision: 29 bp distance

Biological Source:

Source Organism(s):

Escherichia coli (Taxon ID: 562)
synthetic construct (Taxon ID: 32630)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.87 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

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Macromolecular Entities

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoA
Chain IDs:A, B
Chain Length:329
Number of Molecules:2
Biological Source:Escherichia coli

UniProt ID:P0A7Z4 EC:2.7.7.6

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoB
Chain IDs:C
Chain Length:1342
Number of Molecules:1
Biological Source:Escherichia coli

UniProt ID:P0A8V2 EC:2.7.7.6

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoC
Chain IDs:D
Chain Length:1415
Number of Molecules:1
Biological Source:Escherichia coli

UniProt ID:P0A8T7 EC:2.7.7.6

Structures with similar UniProt ID

Structural Superimposition Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoZ
Chain IDs:E
Chain Length:91
Number of Molecules:1
Biological Source:Escherichia coli

UniProt ID:P0A800 EC:2.7.7.6

Polymer Type:polydeoxyribonucleotide
Molecule:Non-template strand homopolym
Chain IDs:H (auth: N)
Chain Length:33
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polyribonucleotide
Molecule:RNA strand homopolymer
Chain IDs:F (auth: R)
Chain Length:13
Number of Molecules:1
Biological Source:synthetic construct

Polymer Type:polydeoxyribonucleotide
Molecule:Template strand homopolymer
Chain IDs:G (auth: T)
Chain Length:32
Number of Molecules:1
Biological Source:synthetic construct

Ligand Molecules

Primary Citation

Structural principles of transcriptional collisions

Watters, J.W, Alushin, G.M, Liu, S, Mueller, A.U, Ju, X, Chuquimarca, S.J, Ye, H.J, Darst, S.A.Show

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Primary Citation of related structures

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