10MJ image
Deposition Date 2026-01-27
Release Date 2026-06-24
Last Version Date 2026-07-15
Entry Detail
PDB ID:
10MJ
Keywords:
TRANSCRIPTION
Title:
Open Mtb-EC: Cryo-EM structure of Mtb RNAP elongation complex (substrate loading mimic) with an open active site (open TL and RH-FL)
Biological Source:
Source Organism(s):
Mycobacterium tuberculosis (Taxon ID: 1773)
Escherichia coli (Taxon ID: 562)
Expression System(s):
Escherichia coli
Method Details:
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
3.30 Å
Aggregation State:
PARTICLE
Reconstruction Method:
SINGLE PARTICLE
10MJ validation report missing
Macromolecular Entities
Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Chain IDs:A, B
Chain Length:347
Number of Molecules:2
Biological Source:Mycobacterium tuberculosis
Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Chain IDs:G (auth: C)
Chain Length:1178
Number of Molecules:1
Biological Source:Mycobacterium tuberculosis
Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Chain IDs:H (auth: D)
Chain Length:1318
Number of Molecules:1
Biological Source:Mycobacterium tuberculosis
Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Chain IDs:C (auth: E)
Chain Length:110
Number of Molecules:1
Biological Source:Mycobacterium tuberculosis
Polymer Type:polydeoxyribonucleotide
Molecule:Template DNA
Chain IDs:E (auth: P)
Chain Length:54
Number of Molecules:1
Biological Source:Escherichia coli
Polymer Type:polydeoxyribonucleotide
Molecule:Non-template DNA
Chain IDs:D (auth: Q)
Chain Length:54
Number of Molecules:1
Biological Source:Escherichia coli
Polymer Type:polyribonucleotide
Molecule:RNA
Chain IDs:F (auth: R)
Chain Length:20
Number of Molecules:1
Biological Source:Escherichia coli
Ligand Molecules
MG
ZN
Primary Citation
RNA polymerase inhibitors reveal active-site motions essential for the nucleotide addition cycle.
Proc.Natl.Acad.Sci.USA 123 e2609228123 e2609228123 (2026)
PMID: 42378287 DOI: 10.1073/pnas.2609228123
Primary Citation of related structures
10MJ
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