ISDA: 10MC

Deposition Date 2026-01-27

Release Date 2026-04-29

Last Version Date 2026-04-29

Entry Detail

PDB ID:

10MC

Keywords:

Transcription/DNA/RNA

Title:

Open2 Eco-ePEC: Cryo-EM structure of Eco RNAP his-elemental paused elongation complex with an open active site (open TL, SI3 and RH-FL)

Biological Source:

Source Organism(s):

Escherichia coli (Taxon ID: 562)

Expression System(s):

Escherichia coli

Method Details:

Experimental Method:

ELECTRON MICROSCOPY

Resolution:

2.80 Å

Aggregation State:

PARTICLE

Reconstruction Method:

SINGLE PARTICLE

Download Validation Report

Macromolecular Entities

Polymer Type:polydeoxyribonucleotide
Molecule:non-template DNA
Chain IDs:D (auth: A)
Chain Length:32
Number of Molecules:1
Biological Source:Escherichia coli

Polymer Type:polydeoxyribonucleotide
Molecule:template DNA
Chain IDs:A (auth: B)
Chain Length:32
Number of Molecules:1
Biological Source:Escherichia coli

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoA
Chain IDs:E (auth: G), F (auth: H)
Chain Length:329
Number of Molecules:2
Biological Source:Escherichia coli

UniProt ID:P0A7Z4 EC:2.7.7.6

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoB
Chain IDs:G (auth: I)
Chain Length:1342
Number of Molecules:1
Biological Source:Escherichia coli

UniProt ID:P0A8V2 EC:2.7.7.6

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoC
Chain IDs:H (auth: J)
Chain Length:1407
Number of Molecules:1
Biological Source:Escherichia coli

UniProt ID:P0A8T7 EC:2.7.7.6

Structures with similar UniProt ID

Protein Blast

Polymer Type:polypeptide(L)
Molecule:DNA-directed RNA polymerase s
Gene (Uniprot):rpoZ
Chain IDs:B (auth: K)
Chain Length:91
Number of Molecules:1
Biological Source:Escherichia coli

UniProt ID:P0A800 EC:2.7.7.6

Polymer Type:polyribonucleotide
Molecule:RNA
Chain IDs:C (auth: R)
Chain Length:19
Number of Molecules:1
Biological Source:Escherichia coli

Ligand Molecules