ELECTRON MICROSCOPY

Sample

Plasmodium vivax Perforin-like Protein2 Pore

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 68218
Reported Resolution (Å) 3.9
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol FLEXIBLE FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type TFS FALCON 4i (4k x 4k)
Electron Dose (electrons/Å2) 40
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 1000
Maximum Defocus (nm) 1600
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 130000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC v4.6.2
PARTICLE SELECTION Topaz v0.2.5
IMAGE ACQUISITION SerialEM 4.2.0
CTF CORRECTION cryoSPARC v4.6.2
MODEL FITTING UCSF Chimera 1.16
MODEL FITTING UCSF ChimeraX 1.7.1
MODEL FITTING Coot 0.9.8
MODEL FITTING ISOLDE 1.6.0
MODEL REFINEMENT PHENIX 1.21_5207
INITIAL EULER ASSIGNMENT cryoSPARC v4.6.2
FINAL EULER ASSIGNMENT cryoSPARC v4.6.2
RECONSTRUCTION cryoSPARC v4.6.2
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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