ELECTRON MICROSCOPY

Sample

B/Hubei-Wujiagang/158/2009 HA in complex with FV2DP1-1B antibody

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 176835
Reported Resolution (Å) 3.09
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol AB INITIO MODEL
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 (6k x 4k)
Electron Dose (electrons/Å2) 46.00
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 400
Maximum Defocus (nm) 1400
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification 105000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4.5.1
IMAGE ACQUISITION EPU 3.6.0.6389
CTF CORRECTION cryoSPARC 4.5.1
MODEL FITTING UCSF ChimeraX 1.10
INITIAL EULER ASSIGNMENT cryoSPARC 4.5.1
FINAL EULER ASSIGNMENT cryoSPARC 4.5.1
CLASSIFICATION cryoSPARC 4.5.1
RECONSTRUCTION cryoSPARC 4.5.1
MODEL REFINEMENT PHENIX 1.14_3260:
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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