ELECTRON MICROSCOPY

Sample

Complex of Leucyl-tRNA synthetase 1 and Isoleucyl-tRNA synthetase

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 135800
Reported Resolution (Å) 3.16
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol RIGID BODY FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details Initial fitting with Chimera followed by optimization using Phenix dock_in_map
Data Acquisition
Detector Type FEI FALCON IV (4k x 4k)
Electron Dose (electrons/Å2) 60
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 800
Maximum Defocus (nm) 2500
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 96000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4.2
IMAGE ACQUISITION cryoSPARC 4.2
CTF CORRECTION cryoSPARC 4.2
MODEL FITTING UCSF Chimera 1.11.12
MODEL FITTING PHENIX 1.18.2
INITIAL EULER ASSIGNMENT cryoSPARC 4.2
FINAL EULER ASSIGNMENT cryoSPARC 4.2
CLASSIFICATION cryoSPARC 4.2
RECONSTRUCTION cryoSPARC 4.2
MODEL REFINEMENT PHENIX 1.18.2
MODEL REFINEMENT Coot 0.8.9
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION Correction was performed following Homogeneous refinement
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