ELECTRON MICROSCOPY

Sample

Poliovirus 3

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE-PROPANE
Sample Vitrification Details 3 ul of sample blotted for 3.5 seconds with -10 blot force on FEI Vitrobot mark IV.
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 56545
Reported Resolution (Å) 1.80
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol RIGID BODY FIT
Refinement Target Cross-correlation coefficient
Overall B Value ?
Fitting Procedure ?
Details Initial model was rigid body fitted using UCSF chimera and Coot. Global minimization and B-factor refinement was performed in real space using phenix_real.space.refine.
Data Acquisition
Detector Type FEI FALCON IV (4k x 4k)
Electron Dose (electrons/Å2) 30
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 800
Maximum Defocus (nm) 2600
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 165000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details Calibrated pixel size was 0.7303 A/pix.
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4.3.1
MODEL REFINEMENT PHENIX 1.21.2_5419
CTF CORRECTION cryoSPARC 4.3.1
INITIAL EULER ASSIGNMENT cryoSPARC 4.3.1
FINAL EULER ASSIGNMENT cryoSPARC 4.3.1
CLASSIFICATION cryoSPARC 4.3.1
RECONSTRUCTION cryoSPARC 4.3.1
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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