ISDA: 9TGM

ELECTRON MICROSCOPY

Sample

MCM2-7 DH bound to Sld3-Sld7

Specimen Preperation

Sample Aggregation State	PARTICLE
Vitrification Instrument	FEI VITROBOT MARK IV
Cryogen Name	ETHANE
Sample Vitrification Details	Wait time: 30 s Blotting time: 1.5 s Blot force: +2

3D Reconstruction

Reconstruction Method	SINGLE PARTICLE
Number of Particles	344362
Reported Resolution (Å)	3.6
Resolution Method	FSC 0.143 CUT-OFF
Other Details	For the composite MS map, resolution was estimated with phenix.mtriage, using the unmasked model-map FSC, which gave an FSC(0.143) resolution of 3.6 Angstrom. Final focused refinement particle subsets used to generate composite map (344,362 particles): DH core (66,052 particles) Sld3 MRD1:Mcm6 (63,192 particles) Sld3 MRD2:Mcm4 (166,980 particles) Sld7:Mcm6 (48,138 particles)
Refinement Type
Symmetry Type	POINT

Map-Model Fitting and Refinement

ID	1
Refinement Space	?
Refinement Protocol	?
Refinement Target	?
Overall B Value	?
Fitting Procedure	?
Details	?

Data Acquisition

Detector Type	GATAN K3 (6k x 4k)
Electron Dose (electrons/Å²)	50.4

Imaging Experiment

Date of Experiment	?
Temprature (Kelvin)
Microscope Model	TFS KRIOS
Minimum Defocus (nm)	1000
Maximum Defocus (nm)	3200
Minimum Tilt Angle (degrees)	?
Maximum Tilt Angle (degrees)	?
Nominal CS	2.7
Imaging Mode	BRIGHT FIELD
Specimen Holder Model	FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification	81000
Calibrated Magnification	?
Source	FIELD EMISSION GUN
Acceleration Voltage (kV)	300
Imaging Details	?

Imaging Experiment

Task	Software Package	Version
PARTICLE SELECTION	Topaz	?
MODEL REFINEMENT	PHENIX	1.21.2_5419
IMAGE ACQUISITION	EPU	?
CTF CORRECTION	CTFFIND	4.1.14
INITIAL EULER ASSIGNMENT	RELION	4.0
FINAL EULER ASSIGNMENT	cryoSPARC	4.6.2
CLASSIFICATION	cryoSPARC	4.6.2
RECONSTRUCTION	cryoSPARC	4.6.2
RECONSTRUCTION	RELION	4.0
RECONSTRUCTION	PHENIX	1.21.2_5419

Image Processing

CTF Correction Type	CTF Correction Details	Number of Particles Selected	Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION	?