ELECTRON MICROSCOPY

Sample

ARISC(E33A)-RAP80 in complex with K63-linked ubiquitin chains

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details blot force = 1 N; blot time = 6 s; waiting time = 27 s
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 214650
Reported Resolution (Å) 2.92
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type TFS FALCON 4i (4k x 4k)
Electron Dose (electrons/Å2) 45.5
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 900
Maximum Defocus (nm) 3000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 165000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION crYOLO v1.6.1
CTF CORRECTION cryoSPARC v4.5.3
MODEL FITTING UCSF ChimeraX v1.6.1
MODEL REFINEMENT PHENIX 1.21.1_5286:
INITIAL EULER ASSIGNMENT cryoSPARC v4.5.3
FINAL EULER ASSIGNMENT cryoSPARC v4.5.3
CLASSIFICATION cryoSPARC v4.5.3
RECONSTRUCTION cryoSPARC v4.5.3
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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