ELECTRON MICROSCOPY

Sample

Dextran utilisome of Bacteroides thetaiotaomicron, with a catalytic-inactive GHdex, in complex with dextran.

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details Added dextran 1.5 & 5 (0.5 mM) and Fluorinated Octyl Maltoside at 0.05% (CMC)
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 116104
Reported Resolution (Å) 2.5
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 (6k x 4k)
Electron Dose (electrons/Å2) 23.1
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 1000
Maximum Defocus (nm) 2000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details Krios recollection of a grid previously collected Glacios grid. Grid squares previously collected were excluded.
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC ?
RECONSTRUCTION cryoSPARC ?
MODEL REFINEMENT PHENIX 1.20.1_4487
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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