ELECTRON MICROSCOPY

Sample

high-affinity choline transporter in DDM with Na+

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 285470
Reported Resolution (Å) 2.83
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol FLEXIBLE FIT
Refinement Target ?
Overall B Value 77.74
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 BIOCONTINUUM (6k x 4k)
Electron Dose (electrons/Å2) 60.6
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 1000
Maximum Defocus (nm) 1600
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 105000
Calibrated Magnification ?
Source LAB6
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC v4.4
PARTICLE SELECTION Topaz ?
IMAGE ACQUISITION EPU 3.8.0.7600
CTF CORRECTION cryoSPARC v4.4
MODEL FITTING UCSF ChimeraX 1.6rc202304202331
INITIAL EULER ASSIGNMENT cryoSPARC v4.4
FINAL EULER ASSIGNMENT cryoSPARC v4.4
CLASSIFICATION cryoSPARC v4.4
RECONSTRUCTION cryoSPARC v4.4
MODEL REFINEMENT PHENIX 1.21.1_5286
MODEL REFINEMENT Coot 0.9.4.1
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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