ELECTRON MICROSCOPY

Sample

Amyloid fibril formed by the aggregation of a 12 aa long fragment from cruciferin 3 (223-234) from A. thaliana.

Specimen Preperation
Sample Aggregation State FILAMENT
Vitrification Instrument LEICA PLUNGER
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method HELICAL
Number of Particles 134959
Reported Resolution (Å) 2.96
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type HELICAL
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol AB INITIO MODEL
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type FEI FALCON IV (4k x 4k)
Electron Dose (electrons/Å2) 52.29
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 600
Maximum Defocus (nm) 1500
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification 165000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION RELION 5.0-beta
CTF CORRECTION RELION 5.0-beta
INITIAL EULER ASSIGNMENT RELION 5.0-beta
FINAL EULER ASSIGNMENT RELION 5.0-beta
CLASSIFICATION RELION 5.0-beta
RECONSTRUCTION RELION 5
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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