ELECTRON MICROSCOPY

Sample

Ternary complex of the E3 ligase DCAF11 and the helicase DDX18 mediated by GSH-M12

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument LEICA EM GP
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 136583
Reported Resolution (Å) 2.38
Resolution Method FSC 0.143 CUT-OFF
Other Details As in cryosparc
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol OTHER
Refinement Target real space correlation
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type TFS FALCON 4i (4k x 4k)
Electron Dose (electrons/Å2) 52.004
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 600
Maximum Defocus (nm) 2000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 165000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION Topaz 0.2.5a
MODEL REFINEMENT PHENIX 1.21.2_5419+SVN
CTF CORRECTION cryoSPARC 4.5.3
INITIAL EULER ASSIGNMENT cryoSPARC 4.5.3
FINAL EULER ASSIGNMENT cryoSPARC 4.5.3
CLASSIFICATION cryoSPARC 4.5.3
RECONSTRUCTION cryoSPARC 4.6.2
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION As implemented in cryosparc live
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