ELECTRON MICROSCOPY

Sample

Dimer of VcINDY in complex with alpha ketoglutarate

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 290548
Reported Resolution (Å) 2.35
Resolution Method FSC 0.143 CUT-OFF
Other Details C2
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol OTHER
Refinement Target cross-correlation coefficient
Overall B Value 52.28
Fitting Procedure ?
Details Initial local fitting was done using Chimera and then coot was used for ajustment.
Data Acquisition
Detector Type GATAN K3 BIOCONTINUUM (6k x 4k)
Electron Dose (electrons/Å2) 53.11
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 1200
Maximum Defocus (nm) 1600
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 105000
Calibrated Magnification 105000
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION Warp 1.0.9
PARTICLE SELECTION Topaz 0.2.5a
IMAGE ACQUISITION Leginon 3.5
CTF CORRECTION cryoSPARC v 4.4.1
MODEL FITTING UCSF Chimera 1.15
MODEL FITTING Coot 0.9.6
MODEL REFINEMENT PHENIX 1.20.1_4487
INITIAL EULER ASSIGNMENT cryoSPARC v 4.4.1
FINAL EULER ASSIGNMENT cryoSPARC v 4.4.1
CLASSIFICATION cryoSPARC v 4.4.1
RECONSTRUCTION cryoSPARC V 4.4.1
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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