ELECTRON MICROSCOPY

Sample

Human HDAC6 catalytic domain (CD1-2)

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 109995
Reported Resolution (Å) 3.86
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol RIGID BODY FIT
Refinement Target ?
Overall B Value 50
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type FEI FALCON IV (4k x 4k)
Electron Dose (electrons/Å2) 50
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 500
Maximum Defocus (nm) 3500
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC v4.2.0
IMAGE ACQUISITION EPU 3.0.0.4164REL
CTF CORRECTION cryoSPARC v4.2.0
MODEL FITTING UCSF ChimeraX version 1.5
INITIAL EULER ASSIGNMENT cryoSPARC v4.2.0
FINAL EULER ASSIGNMENT cryoSPARC v4.2.0
CLASSIFICATION cryoSPARC v4.2.0
RECONSTRUCTION cryoSPARC v4.2.0
MODEL REFINEMENT PHENIX 1.20.1_4487:
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION CTF parameters were estimated using patchCTF in cryoSPARC.
Feedback Form
Name
Email
Institute
Feedback