ELECTRON MICROSCOPY

Sample

Leishmania tarentolae ATP-dependant RNA helicase PRP22/DHX8 in closed conformation bound to pre-mRNA

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details ?
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 98912
Reported Resolution (Å) 3.51
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K3 BIOQUANTUM (6k x 4k)
Electron Dose (electrons/Å2) 50
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model TFS KRIOS
Minimum Defocus (nm) 500
Maximum Defocus (nm) 2500
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model ?
Nominal Magnification 64000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION cryoSPARC 4.6.0
IMAGE ACQUISITION SerialEM ?
CTF CORRECTION cryoSPARC 4.6.0
MODEL REFINEMENT PHENIX 1.19.1
INITIAL EULER ASSIGNMENT cryoSPARC 4.6.0
FINAL EULER ASSIGNMENT cryoSPARC 4.6.0
CLASSIFICATION cryoSPARC 4.6.0
CLASSIFICATION RELION ?
RECONSTRUCTION cryoSPARC 4.6.0
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
Feedback Form
Name
Email
Institute
Feedback