ELECTRON MICROSCOPY

Sample

Structure of the wild-type Staphylococcus aureus 70S ribosome complexed with clincelin

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details Blotting time 2.5 s Blot force 5 Waiting time 30 s
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 61266
Reported Resolution (Å) 2.98
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol RIGID BODY FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K2 SUMMIT (4k x 4k)
Electron Dose (electrons/Å2) 50
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model FEI TALOS ARCTICA
Minimum Defocus (nm) 400
Maximum Defocus (nm) 1800
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS ?
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification ?
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 200
Imaging Details ?
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION RELION 4.0
IMAGE ACQUISITION SerialEM ?
CTF CORRECTION RELION 4.0
MODEL FITTING UCSF Chimera 1.7
MODEL FITTING ISOLDE 1.7
MODEL FITTING Coot 0.9.8.1
MODEL FITTING PHENIX 1.20.1-4487
MODEL REFINEMENT PHENIX 1.20.1-4487
INITIAL EULER ASSIGNMENT RELION 4.0
FINAL EULER ASSIGNMENT RELION 4.0
CLASSIFICATION RELION 4.0
RECONSTRUCTION RELION 4.0
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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