X-RAY DIFFRACTION

Crystallization Details
Method pH Temprature Details
X-RAY DIFFRACTION ? 293 Free VvWzb crystals were grown in drops containing 1.5 ul of protein solution (14 mg/ml in the buffer 10 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT) and 1.5 ul of reservoir solution (100 mM HEPES pH7.5, 200 mM NaCl, 31% (w/v) PEG 3350)
Unit Cell:
a: 47.315 Å b: 48.741 Å c: 117.316 Å α: 90.000° β: 90.000° γ: 90.000°
Symmetry:
Space Group: P 21 21 21
Crystal Properties:
Matthew's Coefficient: 2.07 Solvent Content: 40.66
Refinement Statistics
Diffraction ID Structure Solution Method Cross Validation Method Resolution Limit (High) Resolution Limit (Low) Number of Reflections (Observed) Number of Reflections (R-free) Percent Reflections (Observed) R-Work R-Free Mean Isotropic
X-RAY DIFFRACTION MOLECULAR REPLACEMENT THROUGHOUT 1.7100 58.6600 29954 1437 99.4000 0.2000 0.2230 32.5800
Data Collection
Overall
Resolution Limit (High) Resolution Limit (Low) Percent Possible (Observed) R Merge I (Observed) R Sym I (Observed) Net I Over Average Sigma (I) Redundancy Number Reflections (All) Number Reflections (Observed) Observed Criterion Sigma (F) Observed Criterion Sigma (I) B (Isotropic) From Wilson Plot
1.71 58.66 99.5 ? 0.085 19.2 12.6 ? 29957 ? ? ?
Highest Resolution Shell
# Resolution Limits (Low) Resolution Limits (High) Percent Possible (All) Percent Possible (Observed) R-Sym I (Observed) Mean I Over Sigma (Observed) Redundancy Number Unique Reflections (All)
1 1.711 1.740 99.7 ? 1.188 2.3 12.9 ?
Diffraction
Diffraction experiment
Crystal ID Scattering Type Data Collection Temprature Detector Detector Type Details Collection Date Monochromator Protocol
1 100 K ?
Radiation Source
Source Type Wavelength List Synchrotron Site Beamline
SYNCHROTRON SSRF BEAMLINE BL19U1 0.9788 SSRF BL19U1
Software
Software Name Purpose Version
XDS data reduction Jan 31, 2020 (BUILT 20200417)
BUSTER refinement 2.10.2
PDB_EXTRACT data extraction 3.27
Aimless data scaling 0.5.29
PHASER phasing 2.7.17
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