ELECTRON MICROSCOPY

Sample

Ebola virus Zaire (Makona variant) glycoprotein (mucin-deleted) ectodomain bound to human survivor antibody ADI-15878 Fab

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details 3.5s blot on both sides of the grid using Whattman No. 1 filter paper
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 157861
Reported Resolution (Å) 4.14
Resolution Method FSC 0.143 CUT-OFF
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space ?
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type GATAN K2 SUMMIT (4k x 4k)
Electron Dose (electrons/Å2) 60
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model FEI TALOS ARCTICA
Minimum Defocus (nm) 1000
Maximum Defocus (nm) 3500
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification ?
Calibrated Magnification 43478
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 200
Imaging Details Preliminary grid screening was performed manually
Imaging Experiment
Task Software Package Version
PARTICLE SELECTION RELION 2.1
IMAGE ACQUISITION Leginon ?
CTF CORRECTION Gctf ?
INITIAL EULER ASSIGNMENT RELION 2.1
FINAL EULER ASSIGNMENT RELION 2.1
RECONSTRUCTION RELION 2.1
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
PHASE FLIPPING AND AMPLITUDE CORRECTION ?
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