ELECTRON MICROSCOPY

Sample

CMV-STALLED WHEAT GERM 80S-RNC BOUND TO ERF1 AND ABCE1-ADPNP

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details LIQUID ETHANE, VITROBOT MARK 4
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 39309
Reported Resolution (Å) 9.1
Resolution Method ?
Other Details SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2597 (DEPOSITION ID: 12368).
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID
Refinement Space
Refinement Protocol
Refinement Target
Overall B Value
Fitting Procedure
Details
Data Acquisition
Detector Type TVIPS TEMCAM-F416 (4k x 4k)
Electron Dose (electrons/Å2) 0.02
Imaging Experiment
Date of Experiment 2013-02-20
Temprature (Kelvin)
Microscope Model FEI TITAN KRIOS
Minimum Defocus (nm) 1300
Maximum Defocus (nm) 3500
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model .
Nominal Magnification ?
Calibrated Magnification 147136
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 200
Imaging Details ?
Imaging Experiment
Task Software Package Version
MODEL FITTING Coot ?
MODEL FITTING UCSF Chimera ?
PARTICLE SELECTION STARFISH ?
RECONSTRUCTION SPIDER ?
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
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