ELECTRON MICROSCOPY

Sample

F420 REDUCING HYDROGENASE

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK I
Cryogen Name ETHANE
Sample Vitrification Details VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 70, TEMPERATURE- 103, INSTRUMENT- FEI VITROBOT MARK I, METHOD- BLOTTING 2.5 SECONDS BEFORE PLUNGING,
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 26000
Reported Resolution (Å) 3.36
Resolution Method ?
Other Details FRH IS A FRHABG DODECAMER WITH TETRAHEDRAL SYMMETRY. SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2513. (DEPOSITION ID: 12073).
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol FLEXIBLE FIT
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details METHOD--FLEXIBLE REFINEMENT PROTOCOL--CRYO-EM
Data Acquisition
Detector Type FEI FALCON II (4k x 4k)
Electron Dose (electrons/Å2) 22
Imaging Experiment
Date of Experiment 2013-05-27
Temprature (Kelvin)
Microscope Model FEI POLARA 300
Minimum Defocus (nm) 800
Maximum Defocus (nm) 2500
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.2
Imaging Mode BRIGHT FIELD
Specimen Holder Model .
Nominal Magnification 78000
Calibrated Magnification 106000
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details DATA WAS COLLECTED IN MOVIE MODE
Imaging Experiment
Task Software Package Version
MODEL FITTING Coot ?
RECONSTRUCTION RELION ?
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
? EACH IMAGE
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