ELECTRON MICROSCOPY

Sample

Dengue virus serotype 3

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK IV
Cryogen Name ETHANE
Sample Vitrification Details Blotted with filter paper for 2 seconds prior to snap freezing in liquid ethane (FEI VITROBOT MARK IV)
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 6800
Reported Resolution (Å) 6.0
Resolution Method FSC 0.5 CUT-OFF
Other Details (Single particle details: Particles were manually selected.) (Single particle--Applied symmetry: I)
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol FLEXIBLE FIT
Refinement Target real space correlation
Overall B Value ?
Fitting Procedure ?
Details REFINEMENT PROTOCOL--flexible DETAILS--Initially fitted in Chimera, model rebuilt in Coot, refined in NAMD/MDFF
Data Acquisition
Detector Type GATAN ULTRASCAN 4000 (4k x 4k)
Electron Dose (electrons/Å2) 20.4
Imaging Experiment
Date of Experiment 2011-10-27
Temprature (Kelvin)
Microscope Model FEI TITAN KRIOS
Minimum Defocus (nm) 1100
Maximum Defocus (nm) 3500
Minimum Tilt Angle (degrees) 0.0
Maximum Tilt Angle (degrees) 0.0
Nominal CS 2.7
Imaging Mode BRIGHT FIELD
Specimen Holder Model FEI TITAN KRIOS AUTOGRID HOLDER
Nominal Magnification 75000
Calibrated Magnification ?
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
MODEL FITTING Coot ?
MODEL FITTING MDFF ?
MODEL FITTING NAMD ?
MODEL FITTING UCSF Chimera ?
RECONSTRUCTION EMAN 1
RECONSTRUCTION EMAN 2
RECONSTRUCTION MPSA ?
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
. each particle
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