ELECTRON MICROSCOPY

Sample

Ribosome-bound Dom34-Hbs1-GDPNP complex

Specimen Preperation
Sample Aggregation State PARTICLE
Vitrification Instrument FEI VITROBOT MARK I
Cryogen Name ETHANE
Sample Vitrification Details Blotted for 10 seconds before plunging, used 2 layer of filter paper
3D Reconstruction
Reconstruction Method SINGLE PARTICLE
Number of Particles 38400
Reported Resolution (Å) 9.5
Resolution Method ?
Other Details ?
Refinement Type
Symmetry Type POINT
Map-Model Fitting and Refinement
ID 1
Refinement Space REAL
Refinement Protocol ?
Refinement Target ?
Overall B Value ?
Fitting Procedure ?
Details ?
Data Acquisition
Detector Type KODAK SO-163 FILM
Electron Dose (electrons/Å2) 25
Imaging Experiment
Date of Experiment ?
Temprature (Kelvin)
Microscope Model FEI POLARA 300
Minimum Defocus (nm) 1300
Maximum Defocus (nm) 4000
Minimum Tilt Angle (degrees) ?
Maximum Tilt Angle (degrees) ?
Nominal CS 2.26
Imaging Mode BRIGHT FIELD
Specimen Holder Model .
Nominal Magnification 39000
Calibrated Magnification 39000
Source FIELD EMISSION GUN
Acceleration Voltage (kV) 300
Imaging Details ?
Imaging Experiment
Task Software Package Version
MODEL FITTING MDFF ?
RECONSTRUCTION SPIDER ?
Image Processing
CTF Correction Type CTF Correction Details Number of Particles Selected Particle Selection Details
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